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histone3 cell signaling 9715 rabbit  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc histone3 cell signaling 9715 rabbit
    Histone3 Cell Signaling 9715 Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h3+cell+signaling+9715/Histone+H3+Antibody/pm41888273-91-22-23
    Average 97 stars, based on 4296 article reviews
    histone3 cell signaling 9715 rabbit - by Bioz Stars, 2026-10
    97/100 stars

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    Incubation:

    Article Title: SHMT2-mediated mitochondrial serine metabolism drives 5-FU resistance by fueling nucleotide biosynthesis.
    Article Snippet: Subsequently, 20–25 mg of total proteins were loaded on SDS-PAGE gels and transferred to PVDF membranes (BioRad #1704157). .. Membranes were incubated for 1h at room temperature in blocking buffer (5% non-fat dry milk (SantaCruz Biotechnology #sc-2325) in PBS-Tween 0.1%), and then incubated at 4 C over-night with primary antibodies (against either PHGDH (Thermo Fisher Scientific #PA554360), NRF2 (Genetex #GTX103322), phospho-H2AX (Abcam #81299), H3 (Cell Signaling #9715), p53 (SantaCruz Biotecnology #sc-126), p21 (SantaCruz Biotecnology #sc-271610), MDM2 (SantaCruz Biotecnology #sc-965), tubulin (SantaCruz Biotecnology #sc-5286), GAPDH (SantaCruz Biotecnology #sc-48166), b-Actin (Santa Cruz Biotechology #sc-47778). .. All primary antibodies were diluted 1:1000 in PBS-Tween 0.1% containing 5% BSA (Merck Sigma #A7906)).

    Blocking Assay:

    Article Title: SHMT2-mediated mitochondrial serine metabolism drives 5-FU resistance by fueling nucleotide biosynthesis.
    Article Snippet: Subsequently, 20–25 mg of total proteins were loaded on SDS-PAGE gels and transferred to PVDF membranes (BioRad #1704157). .. Membranes were incubated for 1h at room temperature in blocking buffer (5% non-fat dry milk (SantaCruz Biotechnology #sc-2325) in PBS-Tween 0.1%), and then incubated at 4 C over-night with primary antibodies (against either PHGDH (Thermo Fisher Scientific #PA554360), NRF2 (Genetex #GTX103322), phospho-H2AX (Abcam #81299), H3 (Cell Signaling #9715), p53 (SantaCruz Biotecnology #sc-126), p21 (SantaCruz Biotecnology #sc-271610), MDM2 (SantaCruz Biotecnology #sc-965), tubulin (SantaCruz Biotecnology #sc-5286), GAPDH (SantaCruz Biotecnology #sc-48166), b-Actin (Santa Cruz Biotechology #sc-47778). .. All primary antibodies were diluted 1:1000 in PBS-Tween 0.1% containing 5% BSA (Merck Sigma #A7906)).

    Staining:

    Article Title: RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation
    Article Snippet: Briefly, cells were lysed in RIPA buffer (Santa Cruz Biotechnology) for RNA-seq samples, or nuclear lysis buffer for NET-seq samples on days 8 and/or 11 of differentiation to confirm that cells maintained their SPT5 KD during erythropoiesis. .. Samples were analyzed using antibodies against SPT5 (Millipore, MABE1803), Polymerase II RPB1 (BioLegend, 8WG16), and H3 (Cell Signaling, 9715), and stained with LI-COR secondary antibodies. .. Blots were imaged on the ChemiDoc Imaging System (Bio-Rad).

    Article Title: RNA polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation.
    Article Snippet: Immunoblotting was performed as previously described.107 Briefly, cells were lysed in RIPA buffer (Santa Cruz Biotechnology) for RNA-seq samples, or nuclear lysis buffer for NET-seq samples on days 8 and/or 11 of differentiation to confirm that cells maintained their SPT5 KD during erythropoiesis. .. Samples were analyzed using antibodies against SPT5 (Millipore, MABE1803), Polymerase II RPB1 (BioLegend, 8WG16), and H3 (Cell Signaling, 9715), and stained with LI-COR secondary antibodies. .. Blots were imaged on the ChemiDoc Imaging System (Bio-Rad).



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